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Home > Liquid Chromatography Lab Activity (ScholAR)
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Product Information
Aligned with AP Chemistry Lab #18
Introduce your students to the study of separation and elutants through column chromatography. In the stationary phase, a solid adsorbent is placed in a vertical column. In the mobile phase, a liquid, is added to the top of the column and flows down through the column by either gravity or external pressure. The mixture to be analyzed is applied to the top of the column. The liquid solvent or eluent is passed through the column by gravity or by the application of air pressure. An equilibrium is established between the solute adsorbed on the adsorbent and the eluting solvent flowing down through the column. Because the different components in the mixture have different interactions with the stationary and mobile phases, they will be carried along with the mobile phase to varying degrees and a separation will be achieved. The individual components, or elutants, are collected as the solvent drips from the bottom of the column. Includes materials for 15 setups.
Chemical Contents: Dye mixture Methanol Silica
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