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Teacher Developed Classroom Tested

Illustrate concepts in electrophoresis with this easy-to-use kit, which lets students separate colorful dyes based on electrical charge and mass. Simply put the gel comb in the chamber, microwave the gel and pour it into the chamber, then load your dyes into the wells using the supplied micropipets. After covering the dyes and gel with running buffer, connect five 9V batteries in sequence to the electrode terminals. In just 45 minutes, you’ll see distinct bands of color formed in each lane, as each dye migrates a different distance toward either the positive or negative electrode. By measuring the migration distance of each dye band, students can calculate both the molecular charge and the base pair equivalent size of each fragment. Includes illustrated teacher’s guide with step-by-step instructions. Note: Five 9V batteries are required, but not included.
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