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Teacher Developed Classroom Tested

A Viral Lab that Smokes!
All American tobacco is infected with the Tobacco Mosaic Virus or TMV. In this lab students isolate naturally occurring TMV in cigarette tobacco then inoculate healthy bean plants (P. vulgaris) with it.
TMV symptoms in the form of necrotic local lesions develop on the leaves where the virus was introduced 1 day after infect. Because cell death is so rapid, the virus is not replicated beyond the initially infected cells and the virus is not transported to adjacent uninfected cells.
Virus crystals can be viewed using a microscope in 1 week+. The lab also demonstrates the inhibitory properties of milk against the virus.
Includes: diatomaceous earth, 5” stirrers, 10- 13 x 100 mm test tubes, sterile applicators, 10- 67mm plastic funnels, 2m cheesecloth, 9 oz. powdered milk, phosphate buffer, 1 lb. pinto beans, 10- 4” pots, 8 lbs. soil, instructions.

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