Kemtec™ Amino Acid Analysis by TLC Lab Activity.
About this item
Explore the Theory of Chromatography.
- Visually determine the unknowns
- Target grades 10-14
Students learn the theory of chromatography; then separate two different unknown amino acid mixtures using thin layer chromatography (TLC) with amino acid standards run alongside the unknowns. Ninhydrin is used as a visualizing agent; then the rate of flow (Rf) values are calculated for each spot identified on the chromatograms. Students are challenged to predict the migration of the amino acids on the TLC after looking up the structures and pKa values of the four amino acid standards. The unknown components are identified by comparing the Rf values from the unknown amino acid mixtures to Rf data obtained from the four known amino acid standards. Designed for 24 students working in six groups of 4.
Ordering information: Kit includes Instructor Manual, Student Manual, Student Data Sheets, Amino Acid Standards, Unknown Amino Acid Mixture I, Unknown Amino Acid Mixture II, Silica Gel Strips, Chromatography Solvent, Toothpicks, Foil to cover Chromatography Chambers, Ninhydrin, Acetone, Spray Bottles. Hair Dryer, 600 mL beakers for chromatography chambers, clear plastic rulers, and standard laboratory supplies are required but not included.
Delivery information: Due to the nature of this item, it cannot be shipped by air under any circumstances. Please select ground shipping at time of checkout.
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Product Details & Documents
Explore the Theory of Chromatography.
- Visually determine the unknowns
- Target grades 10-14
Students learn the theory of chromatography; then separate two different unknown amino acid mixtures using thin layer chromatography (TLC) with amino acid standards run alongside the unknowns. Ninhydrin is used as a visualizing agent; then the rate of flow (Rf) values are calculated for each spot identified on the chromatograms. Students are challenged to predict the migration of the amino acids on the TLC after looking up the structures and pKa values of the four amino acid standards. The unknown components are identified by comparing the Rf values from the unknown amino acid mixtures to Rf data obtained from the four known amino acid standards. Designed for 24 students working in six groups of 4.
Ordering information: Kit includes Instructor Manual, Student Manual, Student Data Sheets, Amino Acid Standards, Unknown Amino Acid Mixture I, Unknown Amino Acid Mixture II, Silica Gel Strips, Chromatography Solvent, Toothpicks, Foil to cover Chromatography Chambers, Ninhydrin, Acetone, Spray Bottles. Hair Dryer, 600 mL beakers for chromatography chambers, clear plastic rulers, and standard laboratory supplies are required but not included.
Delivery information: Due to the nature of this item, it cannot be shipped by air under any circumstances. Please select ground shipping at time of checkout.
Documents
VWR GHS Compliance Letter (US)
Additional Product Details
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Product Information
- IncludesNo. of lab groupsAvailabilityPrice
- 470024-062Amino Acid Analysis by TLC Lab ActivityAmino Acid Analysis by TLC Lab ActivityTeacher's Guide and Student Copymaster6Discontinued-Supplier Number: -
- 470024-304Amino Acid Analysis By TLC Lab Activity, Refill KitSupplier Number: 8-164R
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