CRISPR-DX: Sickle Cell Detection Kit

Learn and practice CRISPR.

• Learn the principles of CRISPR technology, including gRNA design and target specificity
• Validate gRNA sequences using genome databases and bioinformatics tools
• Perform CRISPR-based DNA digestion and analyze results using gel electrophoresis
• Integrate molecular biology concepts with real-world diagnostic applications

The CRISPR-Dx: sickle cell detection kit is designed to introduce students to the application of CRISPR technology for the molecular diagnosis of sickle cell disease (SCD). This hands-on investigation guides students through the complete workflow used in modern genetic testing, from guide RNA (gRNA) design and validation to experimental analysis. In this activity, students assume the role of clinical scientists tasked with identifying which of four simulated patient samples contains the sickle cell mutation. Using CRISPR-based detection and gel electrophoresis, students analyze DNA samples and interpret experimental results to determine the disease status of each case. The kit is enough for 24 students. The streamlined and classroom-friendly protocol allows easy integration into biotechnology, genetics, or molecular biology courses. By combining computational analysis with hands-on experimentation, this lab offers students an engaging and authentic introduction to modern biotechnology practices.