"PCR Lab Activities"

Pre-mixed, and ready to use, this master mix contains DNA polymerase, dNTPs, MgCl₂, and reaction buffers at optimal concentrations for the amplification of DNA via PCR. Its special formulation allows for direct loading of PCR samples into agarose gels as well as tracking the migration during electrophoresis without the need for loading dyes. Simply add template, primers, and nuclease-free water, available separately. Mater Mix reduces risk of contamination. Includes enough for 100 reactions. Note: Can be stored at 4 °C for up to six weeks, or −20 °C for longer.

Introduce Students to PCR Technique with a Basic Procedure

Use PCR to determine if your parrots are male (ZZ) or female (ZW).

Use PCR to determine if your chickens are male (ZZ) or female (ZW).

Use PCR to determine if your emus are male (ZZ) or female (ZW).

Use PCR to genotype your own chickens for the lavender allele that causes pale greyish plumage.

Use PCR to genotype your own chickens for the recessive white allele that causes white plumage.

Use PCR to genotype your own chickens for the presence of the oocyan allele that causes blue eggs.

Use PCR to genotype your own chickens for the frizzle allele that causes curly feathers.

Use PCR to accurately determine the sex of geese, ducks, quail, and other fowl.

Teach fundamental concepts of genetic diversity and real world applications in marine biology.