miniPCR® DNA Glow Lab™: Exploring DNA Structure
- L'article nécessite un contrôle de la température pour le stockage et la livraison avec des frais supplémentaires. Il n'est pas éligible au retour pour des raisons de sécurité et de qualité. Tenez compte des exigences avant d'acheter.
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Product Details & Documents
Move beyond paper models and investigate DNA structure directly!
- Techniques: Micropipetting, fluorescence detection
- Topics: DNA structure, hydrogen bonds, fluorescence, biotechnology
- Time required: Can be completed in one or two 45-minute class periods
- Level: General high school through college
Discover the conditions that make double-stranded DNA denature into single strands, then anneal back together again. In this lab, students will use a fluorescent dye to investigate the conditions that influence DNA structure and its transition from double helix to single strand, and vice versa. Inquiry-based investigations explore how DNA sequence, concentration, and pH affect base pairing through hydrogen bonds.
Required equipment (one per lab group):
- Micropipettes and tips
- Thermal cycler or heat block that can accommodate PCR tubes (ideal set up) or water bath and floaties (workable).
- P51™ Molecular Viewer or other blue light transilluminator (such as blueBox™ or blueGel™)
Storage notes:
- Reagents require refrigerator storage
- DNA binding dye must be protected from light
- Reagents are stable for 6 months when stored in the refrigerator and protected from light
Delivery information: The DNA Glow Lab™ kit contains reagents for 8 lab groups: Three DNA samples for analysis; DNA binding dye; Glow Lab Dilution Buffer 1; Glow Lab Dilution Buffer 2; Microtubes. Note: Reagents are compatible with P51™ Molecular Fluorescence Viewer or other blue light transilluminator (460-480 nm, e.g. blueBox™ or blueGel™).
Spécifications
- Désignation:miniPCR® DNA glow lab™
- No. of Lab Groups:8