miniPCR® BioBits® Central Dogma

Illuminate transcription and translation with the BioBits® cell-free system in this innovative classroom-sized kit!

• Techniques: Micropipetting, fluorescence detection, cell-free technology
• Topics: Central dogma of molecular biology, transcription and translation, protein synthesis, gene expression, fluorescence, biotechnology, synthetic biology
• Time required: 30 minutes during 1 class period, followed by 10 minutes for observation 8 - 72 hours later
• Level: General high school through college

BioBits® pellets are tiny molecular factories that can create proteins without the need for cell culture. When dry, BioBits® pellets are dormant, but they can be activated by simply adding water. Researchers have been using cell-free reactions in their laboratories for years, with applications ranging from novel therapeutic discovery to field diagnostics. Now the BioBits® cell-free system makes this cutting-edge technology accessible to anyone interested in learning molecular biology. With minimal equipment requirements and a quick and straightforward protocol, students will use the BioBits® system to visualize the flow of genetic information and monitor transcription and translation in real-time through fluorescence. This activity serves as an excellent interactive tool for learning molecular biology’s central dogma and exposes students to cutting-edge synthetic biology.

Required equipment (one per lab group):
- Micropipettes and tips
- P51™ Molecular Fluorescence Viewer or other blue light transilluminator with an orange filter (460 - 480 nm, e.g. blueBox™ or blueGel™)

Storage notes:
- Reagents require freezer storage
- Reagents are stable for 6 months when stored in the freezer
- If a freezer is not available, BioBits® pellets are viable for three months when stored in a refrigerator

Delivery information: The kit contains the following reagents for 8 lab groups: 32 BioBits® pellets in 8-tube PCR strips; DNA sample A; DNA sample B; nuclease-free water; Kanamycin.