Aceto-Orcein Stain

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Disposal

This disposal method is published for your convenience. You MUST have checked with your federal, state, and local regulations before using this method and these methods are only applicable for small laboratory sized quantities.

Use a lab apron, chemical resistant gloves and goggles before following the below method to dispose of hazardous waste.

 1. To a large beaker, add a large volume of water in a fume hood.
 2. Add the organic acid to the beaker.
 3. Neutralize the mixture by adding dilute sodium hydroxide or sodium carbonate until the pH is about 7. Stir.
 4. Pour the final solution down the drain with at least a 20-fold excess of water.

Solution Prep

To prepare a Aceto-Orcein Staining solution, add 31 mL of glacial acetic acid and 14 mL of DI water to a beaker. Heat to almost boiling. Add 2 g of orcein and allow it to cool. Dilute to 100 mL with DI water.

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