Fast and robust PCR-based GMO detection from your favorite foods.
- Techniques: Micropipetting, DNA extraction, PCR, gel electrophoresis
- Topics: Genetic engineering, environmental science, plant biotechnology
- Time required: Can be completed in two 45-minute class periods or one 120-minute class period
- Level: General high school through college
Humans have been modifying plants since the dawn of civilization through the domestication of crops. Modern biotechnology and genetic engineering allow scientists and breeders to rapidly confer very specific traits by introducing particular genes directly into plants. Test for genetically engineered elements from foods and plant tissues using PCR.
Reagents and consumables supplied by user:
- Agarose (electrophoresis grade)
- DNA stain (e.g., GelGreen™)
- Gel electrophoresis buffer (e.g., 1X TBE)
- Distilled or deionized H₂O (to dilute buffer concentrate – not included in Lab Companion Kit)
- Microtubes
- PCR tubes
Required equipment:
- Micropipettes and tips
- Thermal cycler
- Gel electrophoresis and visualization system
Storage notes:
- Reagents require freezer storage
- Reagents are stable for 12 months when stored in the freezer
Accessories information: The learning lab companion kit (catalog no. 470352-026) contains all the consumables (except water) needed for a class of 32 students. These reagents and consumables can also be sourced independently.
Delivery information: The miniPCR GMO Detection Lab kit contains reagents for 8 lab groups: DNA-EZ™ DNA Extraction System: Lysis and Neutralization Solutions; 5X EZ PCR Master Mix, Load-Ready™; GMO Lab Primer Mix; 2 Control DNA samples (GMO and non-GMO); 100 bp DNA Ladder, Load Ready™. Note: Reagents are compatible with blueGel™ or other standard horizontal electrophoresis systems.
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