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Bio-Rad® Comparative Proteomics Kit II: Western Blot Module

Explore Immunodetection

  • Lab can be completed in four 45 minute lab sessions
  • Includes materials for eight setups
  • Must also purchase Bio-Rad Comparative Proteomics Kit 1
Western blotting employs antibodies to identify proteins of interest in complex protein mixtures such as cell extracts and is a cornerstone of biomedical research. For example, it is used as the confirmatory diagnostic test for HIV and mad cow disease. This western blotting activity takes the Bio-Rad protein profiler kit to the next level. Students generate protein profiles from the muscle tissue of closely and distantly related fish and then use the power of western blotting and immunodetection to specifically identify myosin from the hundreds of other muscle proteins in each fish’s profiles. From their protein profiler results, students make educated guesses as to the identities of the proteins in their gels. Via western blotting, antibodies confirm the presence and location of one specific protein, myosin light chain, in each species’ profile. Measurable differences in the molecular weights of the myosin light chain proteins from different species lead students to hypothesize about how these variations relate to evolutionary relationships of the species.

Kit Contents:
Primary antibody (anti-myosin light chain mouse monoclonal), lyophilized
Secondary antibody (goat anti-mouse polyclonal antibody conjugated to horseradish peroxidase, or HRP), lyophilized
HRP color detection reagent A
HRP color detection reagent B
10x Tris/Glycine, 1 L
Nonfat dry milk blocker
10x Phosphate buffered saline (PBS), 100 ml
10% Tween 20, 5 ml
Nitrocellulose sheets, 0.45 μm
Blotting paper sheets
Reagent tubes
Curriculum including teacher’s guide, student manual, and graphic quick guide

Required Accessories Not Included in Kit:
Comparative proteomics kit I: protein profiler module
Ready Gel® or Mini-PROTEAN® TGX™ precast polyacrylamide gels, 15%, 10-well each
Vertical gel electrophoresis chambers
Adjustable micropipet, 2–20 μl
Power supplies
Water bath or dry bath
Rocking platform
Reagent alcohol or ethanol
Fish samples, 5–8 types

See it in action



Item # Description No. of Lab Groups Unit Availability Price Quantity
470234-022 Comparative Proteomics Kit II: Western Blot Module
Hazardous Product
8 Kit of 1 Retrieving Restricted
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