Students transform bacteria using the jellyfish gene using techniques common in most Biotechnology labs.
- Transform bacteria and make them glow by inserting a jellyfish gene
- Turn the gene on and off to study gene regulation
- Extend the lesson and isolate the fluorescent protein with the optional SDS-PAGE Extension kit (available separately)
In the pGLO™ Bacterial Transformation Kit, students use the pGLO Bacterial Transformation Kit to transfer pGLO plasmid encoding GFP into E. coli, a common prokaryotic organism used for DNA propagation and protein expression. Colonies of E. coli are qualitatively examined for fluorescence to determine whether the pGLO gene is being expressed. Time required for this step is two, 45 min. sessions.
In the pGLO™ Kit SDS-PAGE Extension Activity, gel electrophoresis (SDS-PAGE) is employed to separate the entire repertoire of proteins expressed in E. coli, including the foreign GFP responsible for transferring the fluorescence trait. Time required for this step is three, 45 min. sessions.
Total time to perform BOTH Transformation and SDS-PAGE extension kits is five, 45 min. sessions.
Ordering information: Transformation Kit includes plasmid (lyophilized) and bacteria (lyophilized), and all other reagents needed for transformation, petri dishes, inoculation loops, tubes, floats, transfer pipettes and UV pen light.
SDS-PAGE Extension includes sample buffer, standards, DTT, electrophoresis buffer, protein stain, instructions.