Ward's® DNA Amplification by PCR Lab Activity

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Avg. Ratings: 3.0 (1 Review)

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Avg. Ratings: 3.0 out of 5

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Professor Biology / Professor / PENNSYLVANIA
Yes, I recommend
I used this lab a few years back in majors bio and it worked well. I will use it in my genetics class this spring as an "easy intro lab" as it is a bit simple. What would make it great is having a lambda phage deletion mutation between all or some of the primers. I do not know if such a mutant exists. If you had this the lab would be a fantastic tool Just a thought. The other thing that would be nice is a "refill kit" that does not have the agarose, running buffer, etc.
470024-478KT 356.29 CAD
470024-478
Ward's® DNA Amplification by PCR Lab Activity
Educational Materials Biology Educational Materials PCR Learning Activities
Introduce Students to PCR Technique with a Basic Procedure

  • Reliable PCR reaction
  • Materials for 24 PCR reactions
  • Time Required: ~60 min. plus 2 hrs. for PCR
In this investigation, students amplify various segments of the bacteriophage lambda genome, then electrophorese and analyze the PCR samples. Students use the sizes of the PCR products generated by the three different forward primers and one reverse primer, provided, to map the locations of the primers relative to one another in the bacteriophage lambda genome. You will get enough materials to run up to 24 PCR reactions, as well as a teacher’s guide and student copymaster.

Ordering information: Kit includes 5 mL of sterile Mineral Oil; 5 mL of sterile Water; 500mL of 5X TBE Running Buffer; 0.5 mL vial of Loading Dye; 200ml of 2% Agarose Gel; 24 X 0.5 mLPlastic Reaction Tubes; Floating Rack; 60mL bottle of Ward’s QUIKView DNA Stain Concentrate; biohazard bag; coupon for perishable material. Materials needed but not provided are electrophoresis Chamber; power supply; water bath or thermal cycler; micropipettes; marking pens;; timer; UV Transilluminator; UV Goggles; and ice. Coupon is included for redemption of perishable materials. Redeem by mail, phone, fax, or email.



   
 

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