For AP Biology Classes
- Testing for genetic engineering
- Time required: ~75 min plus 2 hours for PCR
Some foods contain raw materials from genetically modified organisms (GMO). Examples include tofu, corn flakes and corn meal. In this experiment, your students will extract DNA from food or plant material and perform PCR to determine if any GM indicator genes are present. Amplified DNA is separated and sized by agarose gel electrophoresis. (Requires 1 to 3 day shipping)
Kit includes: instructions, DNA extraction reagents, PCR EdvoBeads™, microtubes, primers, DNA ladder, ultrapure water, wax beads, gel loading dye, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain.
Required, but not included: micropipettes to measure between 5 and 50 µl, tips, water bath, microcentrifuge, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator.