Making the Cut with CRISPR CAS-9

How is CRISPR used in biotechnology?

• Interactive modeling
• How does CAS-9 'Make The Cut'
• Reusable

Model how the CAS-9 protein is programmed with RNA to search for and identify specific sequences of DNA before making the double-stranded cut. Explore and discuss how CAS-9 is being engineered with new features that make it a useful tool for editing the human genome.

With making the cut with CRISPR-CAS9's colorful foam pieces and placemat your students can:
Build a schematic model of the CRISPR-CAS-9 RNA-guided endonuclease, including the viral double-stranded DNA and dual guide RNA.
Understand how the CAS-9 protein binds to a specific sequence of viral double-stranded DNA at a PAM site - and cuts it.
Demonstrate how that cut destroys viral genome and prevents the infection of bacteria.
And much more!